AMP: A Science-driven Web-based Application for the TeraGrid

The Asteroseismic Modeling Portal (AMP) provides a web-based interface for astronomers to run and view simulations that derive the properties of Sun-like stars from observations of their pulsation frequencies. In this paper, we describe the architecture and implementation of AMP, highlighting the lightweight design principles and tools used to produce a functional fully-custom web-based science application in less than a year. Targeted as a TeraGrid science gateway, AMP's architecture and implementation are intended to simplify its orchestration of TeraGrid computational resources. AMP's web-based interface was developed as a traditional standalone database-backed web application using the Python-based Django web development framework, allowing us to leverage the Django framework's capabilities while cleanly separating the user interface development from the grid interface development. We have found this combination of tools flexible and effective for rapid gateway development and deployment.Comment: 7 pages, 2 figures, in Proceedings of the 5th Grid Computing Environments Worksho

Lamin A/C dysregulation contributes to cardiac pathology in a mouse model of severe spinal muscular atrophy

Cardiac pathology is emerging as a prominent systemic feature of spinal muscular atrophy (SMA), but little is known about the underlying molecular pathways. Using quantitative proteomics analysis, we demonstrate widespread molecular defects in heart tissue from the Taiwanese mouse model of severe SMA. We identify increased levels of lamin A/C as a robust molecular phenotype in the heart of SMA mice and show that lamin A/C dysregulation is also apparent in SMA patient fibroblast cells and other tissues from SMA mice. Lamin A/C expression was regulated in vitro by knockdown of the E1 ubiquitination factor ubiquitin-like modifier activating enzyme 1, a key downstream mediator of SMN-dependent disease pathways, converging on β-catenin signaling. Increased levels of lamin A are known to increase the rigidity of nuclei, inevitably disrupting contractile activity in cardiomyocytes. The increased lamin A/C levels in the hearts of SMA mice therefore provide a likely mechanism explaining morphological and functional cardiac defects, leading to blood pooling. Therapeutic strategies directed at lamin A/C may therefore offer a new approach to target cardiac pathology in SMA

Predicted and in situ performance of a solar air collector incorporating a translucent granular aerogel cover

This is the post-print version of the Article. The official published version can be accessed from the link below - Copyright @ 2012 ElsevierThere is an opportunity to improve the efficiency of flat plate solar air collectors by replacing their conventional glass covers with lightweight polycarbonate panels filled with high performance aerogel insulation. The in situ performance of a 5.4m2 solar air collector containing granular aerogel is simulated and tested. The collector is incorporated into the external insulation of a mechanically ventilated end terrace house, recently refurbished in London, UK. During the 7 day test period, peak outlet temperatures up to 45 °C are observed. Resultant supply and internal air temperatures peak at 25–30 and 21–22 °C respectively. Peak efficiencies of 22–36% are calculated based on the proposed design across a range of cover types. Measured outlet temperatures are validated to within 5% of their predicted values. Estimated outputs range from 118 to 166 kWh/m2/year for collectors with different thickness granular aerogel covers, compared to 110 kWh/m2/year for a single glazed collector, 140 k h/m2/year for a double glazed collector and 202 kWh/m2/year for a collector incorporating high performance monolithic aerogel. Payback periods of 9–16 years are calculated across all cover types. An efficiency up to 60% and a payback period as low as 4.5 years is possible with an optimised collector incorporating a 10 mm thick granular aerogel cover.This work is supported by the EPSRC, Brunel University, Buro Happold Ltd. and the Technology Strategy Board

A non-myeloablative chimeric mouse model accurately defines microglia and macrophage contribution in glioma.

Resident and peripherally-derived glioma associated microglia/macrophages (GAMM) play a key role in driving tumour progression, angiogenesis, invasion, and attenuating host immune responses. Differentiating these cells' origins is challenging and current pre-clinical models such as irradiation-based adoptive transfer, parabiosis and transgenic mice have limitations. We aimed to develop a novel non-myeloablative transplantation (NMT) mouse model that permits high levels of peripheral chimerism without blood-brain barrier (BBB) damage or brain infiltration prior to tumour implantation.NMT dosing was determined in C57BL/6J or Pep3/CD45.1 mice conditioned with concentrations of busulfan ranging from 25mg/kg to 125mg/kg. Donor haematopoietic cells labelled with eGFP or CD45.2 were injected via tail vein. Donor chimerism was measured in peripheral blood, bone marrow and spleen using flow cytometry. BBB integrity was assessed with anti-IgG and anti-fibrinogen antibodies. Immunocompetent chimerised animals were orthotopically implanted with murine glioma GL-261 cells. Central and peripheral cell contributions were assessed using immunohistochemistry and flow cytometry. GAMM subpopulation analysis of peripheral cells was performed using Ly6C/MHCII/MerTK/CD64.NMT achieves >80% haematopoietic chimerism by 12 weeks without BBB damage and normal life span. Bone marrow derived cells (BMDC) and peripheral macrophages accounted for approximately 45% of the GAMM population in GL-261 implanted tumours. Existing markers such as CD45 high/low proved inaccurate to determine central and peripheral populations while Ly6C/MHCII/MerTK/CD64 reliably differentiated GAMM subpopulations in chimerised and unchimerised mice.NMT is a powerful method for dissecting tumour microglia and macrophage subpopulations and can guide further investigation of BMDC subsets in glioma and neuro-inflammatory diseases. This article is protected by copyright. All rights reserved

Mould and Winter Indoor Relative Humidity in Low Income Households in England

This paper examines the extent to which variation in heating-season, indoor relative humidity (RH) and mould occurrence in English households is explained by dwelling and household characteristics. It is based on analysis of data from a national study of England's Home Energy Efficiency scheme ( Warm Front) which provides grants for energy efficiency improvements to vulnerable households. Surveys were undertaken of dwellings and households participating in the scheme in five urban areas. Half-hourly living room and main bedroom temperatures and RH measurements were recorded for two to four weeks in a subset of dwellings (no.=1604) over the winters of 2001–02 and 2002-03. For each dwelling, regression of indoor vapour pressure excess on outdoor temperature was used to obtain estimates of daily living room and bedroom indoor vapour pressure under standardised conditions (outdoor temperature of 5°C and 80% RH), from which standardised values of indoor relative humidity were derived. We present evidence on the relationship between mould severity and standardised relative humidity, and between both these parameters and household and dwelling characteristics, including Warm Frontimprovements. </jats:p